VemoZyme F 10000NTP – thermostable phytase enzyme
Phytases belong to the class and subclass of hydrolases phosphatases that catalyze the hydrolysis of monoesters of phosphoric acid. Phytase performed stepwise cleavage of orthophosphate ions of phytic acid to form as intermediates in the penta-, tetra-, tri-, di-, and inositol monophosphates (Nayani NR, Markakis P., 1986).
In accordance with the International Nomenclature of enzymes IUPAC-IUBMB three types of phytases: 3-phytase, 5-phytase and 4/6-phytase. Phytase types begin the transformation of phytic acid hydrolysis of an ester bond at different carbon atoms inositol ring, resulting in the formation of various isomers lower inozitolphospate. Phytate hydrolysis mechanism and the structure of the protein molecules of the same type phytases may vary. Phytase of the same type can be attributed to the acidic, neutral or alkaline phosphatase, according to the optimal pH level.
Phytase (myo-inositol-1,2,3,4,5,6-geksakisfosfat-fosfogidrolazy) – a group of enzymes belonging to the subclass of phosphatases carrying release of at least one phosphate ion from the molecule of phytic acid. Hydrolysis of phytic acid formed the lower, ie containing less than six residues of phosphoric acid, inozitolfosfaty, inositol and inorganic phosphate, as well as associated with phytates released cations.
First phytase activity was found in rice bran (Suzuki U. et. Al., 1907) and calf blood (McCollum EV, Hart EB, 1908), ie, in the variety of living organisms. Later phytase were found in bacteria and fungi, including yeast. Consider that in the digestive secretions monogastric animals, including humans, no phytase and phytate hydrolysis is carried out under the influence of enzymes and microflora acidic environment of the stomach. Today we know a lot of enzymes with phytase activity, a growing number (Haefner S. et. Al. [1], 2005; Oh BC et. Al., 2004; Vats P., Banerjee UC, 2004)
Most (about two thirds) of the total phosphorus in plant feed is presented in the form of salts of phytic acid – phytates (Simons PCM et. Al., 1990). Phytase carries both synthesis and hydrolysis of phytic acid. Due to the inability of livestock and poultry produce endogenous phytase, phosphorus, calcium, protein and other related phytic acid nutrients become less available. For the management of potential nutrient feed and more cost-effective and environmentally friendly production of meat and poultry should be used microbial phytase. Enrichment of the diet of microbial phytase makes more available phosphorus, calcium, zinc and copper, improves digestibility and stimulate weight gain. Effective use of microbial phytase dose-dependent, the ratio in the diet of calcium and phosphorus (Ca: P), vitamin D3, composition of the diet, age, and genetic features of animals and birds. [2]